PRODUCTION OF LEUKEMIA INHIBITORY FACTOR BY HUMAN ARTICULAR CHONDROCYTES AND CARTILAGE IN RESPONSE TO INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR-ALPHA

被引：38

作者：

CAMPBELL, IK ^{[1
]}

WARING, P ^{[1
]}

NOVAK, U ^{[1
]}

HAMILTON, JA ^{[1
]}

机构：

[1] ROYAL MELBOURNE HOSP, WALTER & ELIZA HALL INST MED RES, PARKVILLE, VIC 3050, AUSTRALIA

来源：

ARTHRITIS AND RHEUMATISM | 1993年 / 36卷 / 06期

关键词：

D O I：

10.1002/art.1780360608

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. To investigate the production of leukemia inhibitory factor (LIF) b Methods. Chondrocytes and cartilage were cultured with and without added cytokines, and the conditioned media assayed for LIF by a specific radioreceptor competition assay. Results. Interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFalpha) stimulated LIF production by chondrocyte monolayers and cartilage. LIF was generally not detected in unstimulated cultures. Northern blot analysis showed increased amounts of LIF messenger RNA in IL-1- and TNFalpha-treated chondrocyte cultures. Conclusion. Chondrocytes, stimulated by IL-1 and/or TNFalpha, are potential contributors to the elevated levels of LIF observed in the synovial fluids of patients with rheumatoid arthritis and other inflammatory arthritides.

引用

页码：790 / 794

页数：5

共 16 条

[11] FATAL SYNDROME IN MICE ENGRAFTED WITH CELLS PRODUCING HIGH-LEVELS OF THE LEUKEMIA INHIBITORY FACTOR [J].

METCALF, D ;

GEARING, DP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (15) :5948-5952

[12] EXPRESSION OF IL-1 GENES IN HUMAN AND BOVINE CHONDROCYTES - A MECHANISM FOR AUTOCRINE CONTROL OF CARTILAGE MATRIX DEGRADATION [J].

OLLIVIERRE, F ;

GUBLER, U ;

TOWLE, CA ;

LAURENCIN, C ;

TREADWELL, BV .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 141 (03) :904-911

[13] LEUKEMIA INHIBITORY FACTOR - A NOVEL BONE-ACTIVE CYTOKINE [J].

REID, IR ;

LOWE, C ;

CORNISH, J ;

SKINNER, SJM ;

HILTON, DJ ;

WILLSON, TA ;

GEARING, DP ;

MARTIN, TJ .

ENDOCRINOLOGY, 1990, 126 (03) :1416-1420

[14] MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) EXPRESSION IN HUMAN ARTICULAR-CARTILAGE - INDUCTION BY PEPTIDE REGULATORY FACTORS AND DIFFERENTIAL-EFFECTS OF DEXAMETHASONE AND RETINOIC ACID [J].

VILLIGER, PM ;

TERKELTAUB, R ;

LOTZ, M .

JOURNAL OF CLINICAL INVESTIGATION, 1992, 90 (02) :488-496

[15] LEUKEMIA INHIBITORY FACTOR LEVELS ARE ELEVATED IN SEPTIC SHOCK AND VARIOUS INFLAMMATORY BODY-FLUIDS [J].

WARING, P ;

WYCHERLEY, K ;

CARY, D ;

NICOLA, N ;

METCALF, D .

JOURNAL OF CLINICAL INVESTIGATION, 1992, 90 (05) :2031-2037

[16]

WARING PM, IN PRESS ARTHRITIS R

← 1 2 →