PRODUCTION OF LEUKEMIA INHIBITORY FACTOR BY HUMAN ARTICULAR CHONDROCYTES AND CARTILAGE IN RESPONSE TO INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR-ALPHA

被引：38

作者：

CAMPBELL, IK ^{[1
]}

WARING, P ^{[1
]}

NOVAK, U ^{[1
]}

HAMILTON, JA ^{[1
]}

机构：

[1] ROYAL MELBOURNE HOSP, WALTER & ELIZA HALL INST MED RES, PARKVILLE, VIC 3050, AUSTRALIA

来源：

ARTHRITIS AND RHEUMATISM | 1993年 / 36卷 / 06期

关键词：

D O I：

10.1002/art.1780360608

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. To investigate the production of leukemia inhibitory factor (LIF) b Methods. Chondrocytes and cartilage were cultured with and without added cytokines, and the conditioned media assayed for LIF by a specific radioreceptor competition assay. Results. Interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFalpha) stimulated LIF production by chondrocyte monolayers and cartilage. LIF was generally not detected in unstimulated cultures. Northern blot analysis showed increased amounts of LIF messenger RNA in IL-1- and TNFalpha-treated chondrocyte cultures. Conclusion. Chondrocytes, stimulated by IL-1 and/or TNFalpha, are potential contributors to the elevated levels of LIF observed in the synovial fluids of patients with rheumatoid arthritis and other inflammatory arthritides.

引用

页码：790 / 794

页数：5

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