TYPING OF CLOSTRIDIUM-DIFFICILE BY WESTERN IMMUNOBLOTTING WITH 10 DIFFERENT ANTISERA

被引：15

作者：

KATO, H ^{[1
]}

CAVALLARO, JJ ^{[1
]}

KATO, N ^{[1
]}

BARTLEY, SL ^{[1
]}

KILLGORE, GE ^{[1
]}

WATANABE, K ^{[1
]}

UENO, K ^{[1
]}

机构：

[1] CTR DIS CONTROL,NATL CTR INFECT DIS,HOSP INFECT PROGRAM,ATLANTA,GA 30333

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 02期

关键词：

D O I：

10.1128/JCM.31.2.413-415.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Western blotting (immunoblotting) with antisera against each of 10 reference serogroups was evaluated as a means of typing Clostridium difficile. A total of 164 clinical isolates of C. difficile were tested. Variations in band profiles in each serogroup were used to type isolates into subserogroups. This technique was useful for an epidemiological investigation.

引用

页码：413 / 415

页数：3

共 13 条

[1]

Allen SD, 1991, MANUAL CLIN MICROBIO, P505

[2] COMPARISON OF MEDIA FOR THE ISOLATION OF CLOSTRIDIUM-DIFFICILE FROM FECAL SPECIMENS [J].

BARTLEY, SL ;

DOWELL, VR .

LABORATORY MEDICINE, 1991, 22 (05) :335-338

[3] CHARACTERIZATION OF A NOSOCOMIAL CLOSTRIDIUM-DIFFICILE OUTBREAK BY USING PLASMID PROFILE TYPING AND CLINDAMYCIN SUSCEPTIBILITY TESTING [J].

CLABOTS, CR ;

PETERSON, LR ;

GERDING, DN .

JOURNAL OF INFECTIOUS DISEASES, 1988, 158 (04) :731-736

[4] DETECTION OF SPECIFIC ANTIGENS FOR 10 SEROGROUPS OF CLOSTRIDIUM-DIFFICILE [J].

DELMEE, M ;

AVESANI, V ;

ERNEST, I ;

SURLERAUX, M .

MOLECULAR AND CELLULAR PROBES, 1990, 4 (01) :1-10

[5]

DELMEE M, 1986, J CLIN MICROBIOL, V24, P991

[6] SEROGROUPING OF CLOSTRIDIUM-DIFFICILE STRAINS BY SLIDE AGGLUTINATION [J].

DELMEE, M ;

HOMEL, M ;

WAUTERS, G .

JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 21 (03) :323-327

[7] NOSOCOMIAL CLOSTRIDIUM-DIFFICILE COLONIZATION AND DISEASE [J].

JOHNSON, S ;

CLABOTS, CR ;

LINN, FV ;

OLSON, MM ;

PETERSON, LR ;

GERDING, DN .

LANCET, 1990, 336 (8707) :97-100

[8] ACQUISITION OF CLOSTRIDIUM-DIFFICILE FROM THE HOSPITAL ENVIRONMENT [J].

KAATZ, GW ;

GITLIN, SD ;

SCHABERG, DR ;

WILSON, KH ;

KAUFFMAN, CA ;

SEO, SM ;

FEKETY, R .

AMERICAN JOURNAL OF EPIDEMIOLOGY, 1988, 127 (06) :1289-1294

[9] IMMUNOBLOTS AND PLASMID FINGERPRINTS COMPARED WITH SEROTYPING AND POLYACRYLAMIDE-GEL ELECTROPHORESIS FOR TYPING CLOSTRIDIUM-DIFFICILE [J].

MULLIGAN, ME ;

PETERSON, LR ;

KWOK, RYY ;

CLABOTS, CR ;

GERDING, DN .

JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (01) :41-46

[10] IMMUNOCHEMICAL FINGERPRINTING OF CLOSTRIDIUM-DIFFICILE STRAINS ISOLATED FROM AN OUTBREAK OF ANTIBIOTIC-ASSOCIATED COLITIS AND DIARRHEA [J].

POXTON, IR ;

ARONSSON, B ;

MOLLBY, R ;

NORD, CE ;

COLLEE, JG .

JOURNAL OF MEDICAL MICROBIOLOGY, 1984, 17 (03) :317-324

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