ANALYSIS OF THE ESCHERICHIA-COLI GENOME .5. DNA-SEQUENCE OF THE REGION FROM 76.0-MINUTES TO 81.5-MINUTES

被引：210

作者：

SOFIA, HJ ^{[1
]}

BURLAND, V ^{[1
]}

DANIELS, DL ^{[1
]}

PLUNKETT, G ^{[1
]}

BLATTNER, FR ^{[1
]}

机构：

[1] UNIV WISCONSIN, GENET LAB, MADISON, WI 53706 USA

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 13期

关键词：

D O I：

10.1093/nar/22.13.2576

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The DNA sequence of a 225.4 kilobase segment of the Escherichia coli K-12 genome is described here, from 76.0 to 81.5 minutes on the genetic map. This brings the total of contiguous sequence from the E.coli genome project to 725.1 kb(76.0 to 92.8 minutes). We found 191 putative coding genes (ORFs) of which 72 genes were previously known, and 110 of which remain unidentified despite literature and similarity searches. Seven new genes-arsE, arsF, arsG, treF, xylR, xylG, and xylH-were identified as well as the previously mapped pit and dctA genes. The arrangement of proposed genes relative to possible promoters and terminators suggests 90 potential transcription units. Other features include 19 REP elements, 95 computer-predicted bends, 50 Chi sites, and one grey hole. Thirty-one putative signal peptides were found, including those of thirteen known membrane or periplasmic proteins. One tRNA gene (proK) and two insertion sequences (IS5 and IS150) are located in this segment. The genes in this region are organized with equal numbers oriented with or against replication.

引用

页码：2576 / 2586

页数：11

共 72 条

[51] IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF THE GENE CODING FOR ACETALDEHYDE DEHYDROGENASE-II (ACOD) OF ALCALIGENES-EUTROPHUS [J].