SIMULTANEOUS HPLC ANALYSIS OF THE HYPNOTIC BENZODIAZEPINES NITRAZEPAM, ESTAZOLAM, FLUNITRAZEPAM, AND TRIAZOLAM IN PLASMA

被引：40

作者：

BOUKHABZA, A

LUGNIER, AAJ

KINTZ, P

MANGIN, P

机构：

[1] FAC MED STRASBOURG,INST MED LEGALE,11 RUE HUMANN,F-67085 STRASBOURG,FRANCE

[2] FAC PHARM STRASBOURG,TOXICOL FONDAMENTALE CLIN & MED LAB,F-67084 STRASBOURG,FRANCE

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 1991年 / 15卷 / 06期

关键词：

D O I：

10.1093/jat/15.6.319

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new analytical methodology was developed for simultaneous high-performance liquid chromatographic quantitation of nitrazepam, estazolam, flunitrazepam, and triazolam in plasma. After addition of the internal standard nor-prazepam, biological samples and calibration standards were extracted at basic pH into diethyl ether-methylene chloride (2:1, v/v). The reconstituted extract was separated on a reversed-phase Nova Pak C18 column with acidic buffer (6mM)-acetonitrile-methanol (64:23:13, v/v) as mobile phase. The detection was performed at 242 nm. Within-run and day-to-day precision values were about 2-8%. The method is thus sensitive and reproducible for use in clinical and experimental toxicokinetic studies.

引用

页码：319 / 322

页数：4