ELECTROPHORETIC SEPARATION OF S-POMBE CHROMOSOMES IN POLYACRYLAMIDE SOLUTIONS USING A CONSTANT FIELD

被引：21

作者：

GUSZCZYNSKI, T

CHRAMBACH, A

机构：

[1] Section on Macromolecular Analysis, Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, Bethesda

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)91396-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous electrophoretic separations of megabase (Mb) sized DNA have been achieved in pulsed electric fields, using agarose gel as a matrix. The present study demonstrates separations of Mb sized DNA due to a retardation of migration in proportion to the concentration of uncrosslinked polyacrylamide of 5×106 molecular weight, using a constant electric field. Potentially, the method should be applicable to large DNA in general, greatly reducing the instrumental complexity of such separations and rendering them compartible with capillary electrophoresis apparatus. © 1991.

引用

页码：482 / 486

页数：5

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