HUMAN CYTOCHROME-C-OXIDASE SUBUNIT-VIB - CHARACTERIZATION AND MAPPING OF A MULTIGENE FAMILY

被引：13
|
作者：
CARREROVALENZUELA, RD
QUAN, F
LIGHTOWLERS, R
KENNAWAY, NG
LITT, M
FORTE, M
机构：
[1] OREGON HLTH SCI UNIV,DEPT MED GENET,3181 SW SAM JACKSON PK RD,PORTLAND,OR 97201
[2] OREGON HLTH SCI UNIV,DEPT BIOCHEM,PORTLAND,OR 97201
[3] OREGON HLTH SCI UNIV,VOLLUM INST ADV BIOMED RES,PORTLAND,OR 97201
[4] UNIV OREGON,INST MOLEC BIOL,EUGENE,OR 97403
来源：
GENE | 1991年 / 102卷 / 02期
基金：
英国医学研究理事会;
关键词：
RECOMBINANT DNA; LAMBDA-PHAGE VECTOR; CDNA CLONING; MULTIPLE LOCI; PSEUDOGENES;
D O I：
10.1016/0378-1119(91)90082-M
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We report the isolation and sequence of a human heart cDNA coding for cytochrome c oxidase (COX) subunit VIb (COX VIb). This cDNA extends 50 bp upstream from the region coding for the mature peptide. By Northern analysis, a single transcript of approx. 550 nucleotides (nt) has been identified in six human tissues. Southern analysis of human genomic DNA demonstrates the presence of multiple loci that show high homology to the cDNA. These loci cosegregate with either five or six different human chromosomes in human-rodent somatic cell hybrids. Using the COX6b cDNA, genomic sequences representing two of these loci have been isolated and characterized. The nt sequence analysis suggests that both loci represent COX6b pseudogenes.
引用
收藏
页码：229 / 236
页数：8
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