STUDY OF THE ANTIGENIC STRUCTURE OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS GLYCOPROTEIN-E2 USING RAT MONOCLONAL-ANTIBODIES

被引：0

作者：

RAZUMOV, IA ^{[1
]}

AGAPOV, EV ^{[1
]}

PEREBOEV, AV ^{[1
]}

PROTOPOPOVA, EV ^{[1
]}

LEBEDEVA, SD ^{[1
]}

LOKTEV, VB ^{[1
]}

机构：

[1] VEKTOR SCI IND ASSOC,KOLTSOV,USSR

来源：

VOPROSY VIRUSOLOGII | 1991年 / 36卷 / 01期

关键词：

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A collection of 21 rat hybridomas secreting highly affinic monoclonal antibodies to Venezuelan equine encephalomyelitis (VEE) virus was generated. Using a panel of 15 monoclonal antibodies to glycoprotein E2, the antigenic structure of this protein of VEE strains TC-83 and 230 was studied. A competitive radioimmunoassay suggested a new map of the antigenic structure of glycoprotein E2 in which 5 sites including 11 epitopes of monoclonal antibody binding were distinguished. Antibody to E2-2 site neutralized virus infectivity and blocked hemagglutination test and antibody to E2-3 site could only block hemagglutination. Antibodies to other E2 protein sites lacked any biological activity.

引用

页码：34 / 37

页数：4

共 7 条

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