DNA-MEDIATED TRANSFORMATION OF THE SECONDARILY HOMOTHALLIC BASIDIOMYCETE COPRINUS-BILANATUS

被引：10
作者：
BURROWS, DM
ELLIOTT, TJ
CASSELTON, LA
机构：
[1] QUEEN MARY & WESTFIELD COLL,SCH BIOL SCI,MILE END RD,LONDON E1 4NS,ENGLAND
[2] AFRC,INST HORT RES,LITTLEHAMPTON BN17 6LP,W SUSSEX,ENGLAND
来源：
CURRENT GENETICS | 1990年 / 17卷 / 02期
关键词：
Coprinus bilanatus; Heterologous expression; Transformation;
D O I：
10.1007/BF00312864
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The Coprinus cinereus trp-2 gene which encodes a trifunctional protein of the tryptophan biosynthetic pathway was used to transform a trp-2 mutant of the secondarily homothallic species Coprinus bilanatus. Southern blot analysis confirmed the presence of transforming DNA in the genome of transformants and indicated the presence of tandemly duplicated copies of the plasmid in some of these. Although these two species of Coprinus are regarded as closely related, no cross-hybridisation between the homologous trp-2 genes was detected. © 1990 Springer-Verlag.
引用
收藏
页码：175 / 177
页数：3
相关论文
共 15 条
[1]   DNA-MEDIATED TRANSFORMATION OF THE BASIDIOMYCETE COPRINUS-CINEREUS [J].
BINNINGER, DM ;
SKRZYNIA, C ;
PUKKILA, PJ ;
CASSELTON, LA .
EMBO JOURNAL, 1987, 6 (04) :835-840
[2]   HETEROLOGOUS GENE-EXPRESSION IN THE BASIDIOMYCETE FUNGUS COPRINUS-CINEREUS [J].
CASSELTON, LA ;
HERCE, AD .
CURRENT GENETICS, 1989, 16 (01) :35-40
[3]  
CHALLEN MP, 1989, IN PRESS THEOR APPL, V78
[4]   STRAIN IMPROVEMENT IN THE CULTIVATED MUSHROOM AGARICUS-BISPORUS [J].
ELLIOTT, TJ ;
LANGTON, FA .
EUPHYTICA, 1981, 30 (01) :175-182
[5]   GENETIC RATIOS IN SECONDARILY HOMOTHALLIC BASIDIOMYCETES [J].
ELLIOTT, TJ ;
CHALLEN, MP .
EXPERIMENTAL MYCOLOGY, 1983, 7 (02) :170-174
[6]  
HAMLYN PF, 1981, MICRO TECHNOL, V3, P301
[7]   TRYPTOPHAN BIOSYNTHETIC GENES IN EUKARYOTIC MICROORGANISMS [J].
HUTTER, R ;
NIEDERBERGER, P ;
DEMOSS, JA .
ANNUAL REVIEW OF MICROBIOLOGY, 1986, 40 :55-77
[8]   RAPID AND EFFICIENT COSMID CLONING [J].
ISHHOROWICZ, D ;
BURKE, JF .
NUCLEIC ACIDS RESEARCH, 1981, 9 (13) :2989-2998
[9]   BIFACTORIAL INCOMPATIBILITY IN 2-SPORED BASIDIOMYCETES COPRINUS-SASSII AND C COPRINUS-BILANATUS [J].
KEMP, RFO .
TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1974, 62 (03) :547-555
[10]   GENE CLONING AND TRANSFORMATION IN THE BASIDIOMYCETE FUNGUS COPRINUS-CINEREUS - ISOLATION AND EXPRESSION OF THE ISOCITRATE LYASE GENE (ACU-7) [J].
MELLON, FM ;
LITTLE, PFR ;
CASSELTON, LA .
MOLECULAR AND GENERAL GENETICS, 1987, 210 (02) :352-357
12