ANALYTICAL TECHNIQUES FOR CELL FRACTIONS .13. ROTOR A-16, A PLASTIC GRADIENT-REORIENTING ROTOR FOR ISOLATING NUCLEI

被引：6
作者：
ELROD, LH
PATRICK, LC
ANDERSON, NG
机构：
[1] Molecular Anatomy Program, Oak Ridge National Laboratory2 2 Operated by Union Carbide Corporation Nuclear Div. for the U. S. At, Oak Ridge, TN 37830
来源：
ANALYTICAL BIOCHEMISTRY | 1969年 / 30卷 / 02期
关键词：
D O I：
10.1016/0003-2697(69)90395-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The A-XVI rotor is a simply constructed and easily operated device for making centrifugal separation of whole cells and the larger formed subcellular particles from homogenates. Nuclei were successfully isolated on a sedimentation-rate basis. Studies were made on the effects of reorientation on sucrose gradients. It was shown that, with proper control of acceleration, deceleration, and internal configuration, sucrose gradients can be reoriented without serious degradation. © 1969.
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页码：230 / &
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