PURIFICATION OF PLANT CALMODULIN BY FLUPHENAZINE-SEPHAROSE AFFINITY CHROMATOGRAPHY

被引：236
作者：
CHARBONNEAU, H
CORMIER, MJ
机构：
[1] Bioluminescence Laboratory, Department of Biochemistry University of Georgia, Athens
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 03期
关键词：
D O I：
10.1016/0006-291X(79)91931-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The calcium-dependent binding of phenothiazine drugs to calmodulin (Levin, R. M. and Weiss, B. (1977) Mol. Pharmacol. 13, 690-697) has been utilized to develop a rapid purification procedure for calmodulin based on fluphenazine-Sepharose affinity chromatography. Calmodulin from plant, a fungus, porcine brain and the coelenterate, Renilla reniformis, were easily purified by the calcium-dependent binding of calmodulin to fluphenazine-Sepharose. © 1979.
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页码：1039 / 1047
页数：9
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