DEFINING A NOVEL RIBONUCLEOTIDE REDUCTASE R1 MESSENGER-RNA CIS-ELEMENT THAT BINDS TO AN UNIQUE CYTOPLASMIC TRANS-ACTING PROTEIN

被引：19

作者：

CHEN, FY ^{[1
]}

AMARA, FM ^{[1
]}

WRIGHT, JA ^{[1
]}

机构：

[1] UNIV MANITOBA,MANITOBA INST CELL BIOL,DEPT BIOCHEM & MOLEC BIOL,WINNIPEG R3E 0V9,MB,CANADA

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 22期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1093/nar/22.22.4796

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ribonucleotide reductase is a highly regulated rate-limiting enzyme activity in DNA synthesis, responsible for reducing ribonucleotides to their deoxyribonucleotide forms. Using 3'-end labeled RNA and band-shift and UV cross-linking analyses, we have identified a cis-element, 5'-CAAACUUCS-3', within the 3'-untranslated region of the mammalian ribonucleotide reductase R1 mRNA, which binds a cytoplasmic protein in BALB/c 3T3 mouse cells, to form a 57 kDa RNA-protein complex. Sequence-specific binding was observed, and binding was prevented by several different mutations within the cis-element. We suggest that this cis - trans interaction plays a role in R1 mRNA stability.

引用

页码：4796 / 4797

页数：2

共 19 条

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