A DUAL FUNCTIONAL SIGNAL MEDIATOR SHOWING RHOGAP AND PHOSPHOLIPASE C-DELTA STIMULATING ACTIVITIES

被引：193

作者：

HOMMA, Y ^{[1
]}

EMORI, Y ^{[1
]}

机构：

[1] UNIV TOKYO, FAC SCI, DEPT BIOPHYS & BIOCHEM, BUNKYO KU, TOKYO 113, JAPAN

来源：

EMBO JOURNAL | 1995年 / 14卷 / 02期

关键词：

GTPASE ACTIVATING PROTEIN; PHOSPHOLIPASE C; PIP2; RHO; SIGNAL TRANSDUCTION;

D O I：

10.1002/j.1460-2075.1995.tb07002.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned a novel regulator protein, p122, in the PLC-delta signalling pathway by screening a rat brain expression library with antiserum raised against purified phospholipase C-delta 1 (PLC-delta 1). This novel p122-RhoGAP binds to PLC-delta 1 and activates the phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolyzing activity of PLC-delta 1. As suggested by the deduced amino acid sequence, this regulator protein shows a similarity to the GTPase activating protein (GAP) homology region of Bcr and possesses GAP activity for RhoA, but not for Rac1; no guanine nucleotide exchange activity for RhoA and Rad was detected. These findings suggest that this novel RhoGAP is involved in the Rho signalling pathway, probably downstream of Rho activation, and mediates the stimulation of PLC-delta, which leads to actin-related cytoskeletal changes through the hydrolysis of PIP2, which binds to actin binding proteins such as gelsolin and profilin.

引用

页码：286 / 291

页数：6

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