ASSOCIATION OF GLYCERYLPHOSPHORYLCHOLINE WITH HUMAN SPERM AND EFFECT OF CAPACITATION ON THEIR METABOLISM

被引:6
作者
MITRA, J [1 ]
CHOWDHURY, M [1 ]
机构
[1] INDIAN INST CHEM BIOL,CALCUTTA 700032,W BENGAL,INDIA
关键词
D O I
10.1071/RD9940679
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The presence and localization of glycerylphosphorylcholine (GPC) on the surface of human sperm, as well as the metabolism of its breakdown product L-glycerol 3 phosphate (G3P), were investigated. GPC was found to be associated with sperm after penetrating cervical mucus and was present after repeated washing of the sperm. GPC was partially released by treatment with 0.4 M NaCl in 0.01 M sodium phosphate buffer (pH 7.4) and localized to the head region after sperm fractionation. G3P did not increase O-2 uptake of uncapacitated human sperm. However, under aerobic conditions, lactate accumulated when exogenous G3P or uterine GPC diesterase was added to sperm in suspension. The uptake of O-2 by washed capacitated sperm pre-incubated with 1 unit of rat uterine GPC diesterase for 30 min was significant. This effect was inhibited by 2 mu M oligomycin indicating that oxidative phosphorylation had occurred. The present study indicates that GPC may play a role in the metabolism of human sperm after capacitation.
引用
收藏
页码:679 / 685
页数:7
相关论文
共 34 条
[1]   EFFECTS OF SPERM EXTRACTS AND ENERGY-SOURCES ON MOTILITY AND ACROSOME REACTION OF HAMSTER SPERMATOZOA INVITRO [J].
BAVISTER, BD ;
YANAGIMACHI, R .
BIOLOGY OF REPRODUCTION, 1977, 16 (02) :228-237
[2]   MODE OF ACTION OF ALPHA-CHLOROHYDRIN AS A MALE ANTI-FERTILITY AGENT - INHIBITION OF METABOLISM OF RAM SPERMATOZOA BY ALPHA-CHLOROHYDRIN AND LOCATION OF BLOCK IN GLYCOLYSIS [J].
BROWNWOODMAN, PDC ;
MOHRI, H ;
MOHRI, T ;
SUTER, D ;
WHITE, IG .
BIOCHEMICAL JOURNAL, 1978, 170 (01) :23-37
[3]  
Calamera J C, 1974, Andrologia, V6, P67
[4]   GLYCERYLPHOSPHORYLCHOLINE AND PHOSPHORYLCHOLINE IN SEMEN, AND THEIR RELATION TO CHOLINE [J].
DAWSON, RMC ;
MANN, T ;
WHITE, IG .
BIOCHEMICAL JOURNAL, 1957, 65 (04) :627-634
[5]  
DEJONGE CJ, 1989, J ANDROL, V10, P232
[6]   EFFECT OF ZINC ON HUMAN SPERM RESPIRATION [J].
ELIASSON, R ;
JOHNSEN, O ;
LINDHOLMER, C .
LIFE SCIENCE PART 1 PHYSIOLOGY & PHARMACOLOGY, 1971, 10 (22) :1317-+
[7]  
FORD WCL, 1981, J REPROD FERTIL, V63, P271
[8]  
FRASER LR, 1981, J REPROD FERTIL, V61, P25, DOI 10.1530/jrf.0.0610025
[9]  
FRENKEL G, 1974, FERTIL STERIL, V25, P84
[10]  
HICKS JJ, 1972, FERTIL STERIL, V23, P172