INTERACTION OF IL-1-BETA, IL-6 AND TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) IN HUMAN T-CELLS ACTIVATED BY MURINE ANTIGENS

被引：32

作者：

PANZER, S ^{[1
]}

MADDEN, M ^{[1
]}

MATSUKI, K ^{[1
]}

机构：

[1] UNIV MINNESOTA,IMMUNOBIOL RES CTR,DEPT LAB MED & PATHOL,MINNEAPOLIS,MN 55455

来源：

CLINICAL AND EXPERIMENTAL IMMUNOLOGY | 1993年 / 93卷 / 03期

关键词：

CYTOKINE INTERACTION; T-CELL ACTIVATION;

D O I：

10.1111/j.1365-2249.1993.tb08203.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We used a mixed leucocyte culture between human T cells and irradiated murine splenocytes which allowed us to distinguish between cytokine production from the responder and stimulator cells by the use of species-specific assays for mRNA up-regulation. Using this model of T cell activation by antigen, we studied the effects of human antigen-presenting cell-derived cytokines IL-1beta, IL-6 and TNF-alpha on the activation of human T cell subsets. We show in this system that exogenously added IL-1beta, IL-6 and TNF-alpha induces IL-2 receptor (R) up-regulation and IL-2 production, and proliferation by both CD4+ and CD8+ cells. The addition of IL-1beta induces IL-6 mRNA, and anti-IL-1 antibodies or an IL-IR antagonist protein completely suppresses IL-6 and TNF-alpha supported proliferation. Similarily, addition of IL-6 or TNF-alpha induces up-regulation of IL-1beta mRNA. However, anti-IL-6 and anti-IL-6R antibodies only partially block proliferation supported by IL-1beta. These findings suggest that IL-6 and TNF-alpha will induce IL-2R up-regulation/IL-2 secretion via the induction of IL-1beta production.

引用

页码：471 / 478

页数：8

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