CLONING AND STABLE MAINTENANCE OF 300-KILOBASE-PAIR FRAGMENTS OF HUMAN DNA IN ESCHERICHIA-COLI USING AN F-FACTOR-BASED VECTOR

被引：1269
作者：
SHIZUYA, H
BIRREN, B
KIM, UJ
MANCINO, V
SLEPAK, T
TACHIIRI, Y
SIMON, M
机构：
[1] CALTECH,DIV BIOL,14775,PASADENA,CA 91125
[2] UNIV SO CALIF,DEPT BIOL SCI,LOS ANGELES,CA 90089
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 18期
关键词：
ELECTROPORATION; PHYSICAL MAPPING; HUMAN GENOME;
D O I：
10.1073/pnas.89.18.8794
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA fragments of >300 kilobase pairs. Individual clones of human DNA appear to be maintained with a high degree of structural stability in the host, even after 100 generations of serial growth. Because of high cloning efficiency, easy manipulation of the cloned DNA, and stable maintenance of inserted DNA, the BAC system may facilitate construction of DNA libraries of complex genomes with fuller representation and subsequent rapid analysis of complex genomic structure.
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页码：8794 / 8797
页数：4
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