The enhancement of protein separation by duplex SDS-PAGE

被引：0

作者：

Pyo, Jae Sung ^{[1
]}

Roh, Si Hun ^{[1
]}

Song, Jin-Su ^{[2
]}

Lee, Kyung Hyeon ^{[2
]}

Kim, Hie-Joon ^{[2
]}

Park, Jeong Hill ^{[1
]}

Kwon, Sung Won ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Pharm & Res, Inst Pharmaceut Sci, Seoul 151742, South Korea

[2] Seoul Natl Univ, Dept Chem, Seoul 151742, South Korea

来源：

ANALYTICAL SCIENCE AND TECHNOLOGY | 2006年 / 19卷 / 06期

关键词：

protein separation; SDS-PAGE; MALDI TOF MS;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The protein separation with molecular weight using SDS-PAGE(sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is the one of the most conventional and simple techniques. In, this study, two dimensional SDS-PAGE using same separation principle consecutively was investigated and compared with one dimensional SDS-PAGE. The enhanced separation from duplex SDS-PAGE was observed and separated proteins in the gel were identified by MALDI TOF MS. Identified proteins from different gel spots were found to have different gi numbers. Therefore, duplex SDS-PAGE separation method will be used for economic separation method in the future because only tiny amount of inexpensive reagents are used to perform duplex SDS-PAGE.

引用

页码：529 / 534

页数：6

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