POLIOVIRUS MUTANTS RESISTANT TO NEUTRALIZATION WITH SOLUBLE CELL RECEPTORS

被引：51

作者：

KAPLAN, G ^{[1
]}

PETERS, D ^{[1
]}

RACANIELLO, VR ^{[1
]}

机构：

[1] COLUMBIA UNIV COLL PHYS & SURG,DEPT MICROBIOL,701 W 168TH ST,NEW YORK,NY 10032

来源：

SCIENCE | 1990年 / 250卷 / 4987期

关键词：

D O I：

10.1126/science.2177226

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Poliovirus mutants resistant to neutralization with soluble cellular receptor were isolated. Replication of soluble receptor-resistant (srr) mutants was blocked by a monoclonal antibody directed against the HeLa cell receptor for poliovirus, indicating that the mutants use this receptor to enter cells. The srr mutants showed reduced binding to HeLa cells and cell membranes. However, the reduced binding phenotype did not have a major impact on viral replication, as judged by plaque size and one-step growth curves. These results suggest that the use of soluble receptors as antiviral agents could lead to the selection of neutralization-resistant mutants that are able to bind cell surface receptors, replicate, and cause disease.

引用

页码：1596 / 1599

页数：4

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