A CLONED ALUI-EQUIVALENT REPETITIVE SEQUENCE THAT DETECTS TRANSFECTED HAMSTER DNA

被引：3

作者：

BONE, NM

ARRAND, JE

机构：

[1] MT VERNON HOSP,CANC RES CAMPAIGN GRAY LAB,POB 100,NORTHWOOD HA6 2JR,MIDDX,ENGLAND

[2] UNIV SUSSEX,DEPT BIOL SCI,BRIGHTON BN1 9QG,E SUSSEX,ENGLAND

来源：

GENE | 1990年 / 94卷 / 02期

关键词：

hybridisation probes; recombinant DNA; species-specific DNA; Transfection;

D O I：

10.1016/0378-1119(90)90402-D

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An AluI-equivalent interspersed repetitive element showing 75% homology to a published consensus sequence, which is present at a level of 3 × 105 copies per hamster genome [Haynes et al., Mol. Cell. Biol. 1 (1981) 573-583], has been cloned into plasmid pUC8 as a tandem copy which can be readily excised, self-ligated and labelled for use as a hybridisation probe. This probe successfully detects integrated hamster sequences in the DNA of transfected human cells [Arrand et al., Proc. Natl. Acad. Sci. USA 86 (1989) 6997-7001], whereas repetitive hamster DNA isolated as a C0t = 1 (fast reannealing) fraction fails to do so. Hamster DNA-containing clones in genomic libraries derived from hamster/human hybrids are also easily detected with this probe, thus facilitating the isolation of hamster genes which complement human gene defects. © 1990.

引用

页码：303 / 304

页数：2

共 3 条

[1] MOLECULAR-CLONING AND CHARACTERIZATION OF A MAMMALIAN EXCISION REPAIR GENE THAT PARTIALLY RESTORES UV RESISTANCE TO XERODERMA PIGMENTOSUM COMPLEMENTATION GROUP-D CELLS [J].