PREDICTION OF RETENTION TIMES OF THE NUCLEOSIDES AND BASES ON REVERSE PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY DURING GRADIENT ELUTION

被引：44

作者：

HARTWICK, RA ^{[1
]}

GRILL, CM ^{[1
]}

BROWN, PR ^{[1
]}

机构：

[1] UNIV RHODE ISL,DEPT CHEM,KINGSTON,RI 02881

来源：

ANALYTICAL CHEMISTRY | 1979年 / 51卷 / 01期

关键词：

D O I：

10.1021/ac50037a016

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Through the use of relationships between in k' and composition of the mobile phase, it is possible to predict to within about 5% the retention times of the nucleosides and bases under gradient elution conditions. Through the use of equations, optimal separation of the compounds hypoxanthine, uridine, xanthine, and six other compounds was achieved using reverse phase (C18) columns. The equations predicted the gradient conditions needed to separate these compounds in minimum time with maximum resolution. © 1979, American Chemical Society. All rights reserved.

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页码：34 / 38

页数：5

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