LIGAND-BINDING ANALYSIS OF INTERLEUKIN-2 RECEPTOR COMPLEXES USING SURFACE-PLASMON RESONANCE

被引：3

作者：

WU, ZN

JOHNSON, KW

CIARDELLI, TL

机构：

[1] VET ADM MED CTR,WHITE RIVER JCT,VT 05009

[2] DARTMOUTH COLL,SCH MED,DEPT PHARMACOL & TOXICOL,HANOVER,NH 03755

[3] CHIRON CORP,EMERYVILLE,CA 94608

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 183卷 / 01期

关键词：

SURFACE PLASMON RESONANCE; IL-2; RECEPTOR; CYTOKINE RECEPTOR; COILED COIL; LEUCINE ZIPPER;

D O I：

10.1016/0022-1759(95)00040-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study we have employed surface plasmon resonance to examine the kinetic binding constants of a new class of soluble cytokine receptor complexes. The solution assembly of both homomeric and heteromeric interleukin-2 receptor ectodomain complexes has been achieved using coiled-coil molecular recognition. These complexes were immobilized on a biosensor surface acid the kinetic binding constants were determined. The values obtained for these parameters compared favorably with those reported in studies of comparable cell surface complexes. The combination of these methods provides a powerful approach to the ligand interactions of cytokine receptors.

引用

页码：127 / 130

页数：4

共 11 条

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