IMMUNIZATION OF RABBITS WITH ENTEROTOXIGENIC ESCHERICHIA-COLI COLONIZATION FACTOR ANTIGEN (CFA/I) ENCAPSULATED IN BIODEGRADABLE MICROSPHERES OF POLY (LACTIDE-CO-GLYCOLIDE)

被引：69

作者：

EDELMAN, R ^{[1
]}

RUSSELL, RG ^{[1
]}

LOSONSKY, G ^{[1
]}

TALL, BD ^{[1
]}

TACKET, CO ^{[1
]}

LEVINE, MM ^{[1
]}

LEWIS, DH ^{[1
]}

机构：

[1] MEDISORB TECHNOL INT LP, CINCINNATI, OH 45242 USA

来源：

VACCINE | 1993年 / 11卷 / 02期

关键词：

ENTEROTOXIGENIC ESCHERICHIA-COLI; ORAL VACCINE; FIMBRIAL ADHESIN; MICROSPHERES; BIODEGRADABLE POLYMER;

D O I：

10.1016/0264-410X(93)90012-M

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have searched for an effective oral delivery system for a purified enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin, CFA/I, which elicits anti-colonization immunity. Purified CFA/I antigen encapsulated in biodegradable polymer microspheres of poly (DL-lactide-co-glycolide) (PLG) induced a vigorous, systemic CFA/I IgG antibody response in rabbits immunized once via intragastric tube; oral, unencapsulated CFA/I induced little or no circulating antibody. CFA/I-specific, S-IgA coproantibody was detected in one of three rabbits fed with the CFA/I-PLG microsphere vaccine. We conclude that PLG microspheres protected CFA/I from degradation in the stomach and effectively delivered the antigen for processing by the host's immune system.

引用

页码：155 / 158

页数：4

共 18 条

[1] POLY(DL-LACTIDE-CO-GLYCOLIDE) NORETHISTERONE MICROCAPSULES - AN INJECTABLE BIODEGRADABLE CONTRACEPTIVE [J].