SERUM-FREE AND SERUM-CONTAINING MEDIA FOR HYBRIDOMA CULTURE

被引:0
|
作者
BARMAN, HK [1 ]
RAJPUT, YS [1 ]
机构
[1] NATL DAIRY RES INST,DIV ANIM BIOCHEM,KARNAL 132001,HARYANA,INDIA
来源
关键词
D O I
暂无
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Serum is a complex mixture of large number of constituents differing in molecular weights, chemical nature and functions, and its supplementation is required in media formulations for culturing of hybridomas. Since fetal calf serum is fairly expensive, serum-free (completely defined) media or media formulations with reduced level of fetal calf serum arc being tested for growth of hybridomas and antibody production. Usefulness, limitation, and future scope of these media formulations are discussed.
引用
收藏
页码:803 / 807
页数:5
相关论文
共 50 条
  • [21] Histochemical and ultrastructural evaluations of cytoplasmic lipid droplets in bovine embryos cultured in serum-free and serum-containing media
    Abe, H
    Yamashita, S
    Itoh, T
    Satoh, T
    Hoshi, H
    THERIOGENOLOGY, 1999, 51 (01) : 232 - 232
  • [22] Hydrogen Peroxide-Induced Neurotoxicity in Cultured Cortical Cells Grown in Serum-Free and Serum-Containing Media
    Karina C. Ricart
    Mónica L. Fiszman
    Neurochemical Research, 2001, 26 : 801 - 808
  • [23] Comparative analysis of differentially secreted proteins in serum-free and serum-containing media by using BONCAT and pulsed SILAC
    Shin, Jihye
    Rhim, Jiheon
    Kwon, Yumi
    Choi, Sunyoung
    Shin, Sungho
    Ha, Chul-Won
    Lee, Cheolju
    SCIENTIFIC REPORTS, 2019, 9 (1)
  • [24] SF9 cells infection and Fuc-TIII quality in serum-free and serum-containing media
    Deparis, V
    Cerutti, M
    Goergen, JL
    Marc, A
    ANIMAL CELL TECHNOLOGY: FROM TARGET TO MARKET, 2001, 1 : 91 - 93
  • [25] Hydrogen peroxide-induced neurotoxicity in cultured cortical cells grown in serum-free and serum-containing media
    Ricart, KC
    Fiszman, ML
    NEUROCHEMICAL RESEARCH, 2001, 26 (07) : 801 - 808
  • [26] DIFFERENCES IN METABOLITE UPTAKE BY MDCK CELLS GROWN IN SERUM-CONTAINING AND IN HORMONALLY-DEFINED, SERUM-FREE MEDIA
    YANG, CPH
    COBB, MH
    JEFFERSON, DM
    SCOTT, WN
    FEDERATION PROCEEDINGS, 1980, 39 (06) : 1710 - 1710
  • [27] Comparative analysis of differentially secreted proteins in serum-free and serum-containing media by using BONCAT and pulsed SILAC
    Jihye Shin
    Jiheon Rhim
    Yumi Kwon
    Sun Young Choi
    Sungho Shin
    Chul-Won Ha
    Cheolju Lee
    Scientific Reports, 9
  • [28] Serum-free media for cell culture
    不详
    ATLA-ALTERNATIVES TO LABORATORY ANIMALS, 2004, 32 (01): : 6 - 6
  • [29] Effect of serum-free and serum-containing medium on cellular levels of ER-based proteins in various mouse hybridoma cell lines
    Lambert, N
    Merten, OW
    BIOTECHNOLOGY AND BIOENGINEERING, 1997, 54 (02) : 165 - 180
  • [30] SERUM-FREE MEDIA FOR LYMPHOID CULTURE
    BJARE, U
    JOURNAL OF BIOTECHNOLOGY, 1990, 15 (1-2) : 147 - 154