TETRACYCLINE AS A FLUORESCENT SHELL-MARKER IN THE ABALONE HALIOTIS-IRIS

被引:24
|
作者
PIRKER, JG [1 ]
SCHIEL, DR [1 ]
机构
[1] UNIV CANTERBURY,DEPT ZOOL,PRIVATE BAG 4800,CHRISTCHURCH 1,NEW ZEALAND
关键词
D O I
10.1007/BF00350734
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
Ageing of abalone by counting rings laid down internally in the shell has presented problems of validation. Three methods of tetracycline marking were tested in Haliotis iris. Juvenile (< 70 mm shell length) and adult (> 11 5 mm) abalone were injected intramuscularly with tetracycline hydrochloride, injected with oxytetracycline, or immersed in tetracycline hydrochloride. After treatment, shells were cut sagitally and examined under ultraviolet light using a stereomicroscope. In treatments where juveniles were injected with tetracycline hydrochloride or oxytetracycline hydrochloride at dosages ranging from 20 to 600 mg per kg body weight, no fluorescent marks were visible from treatments less-than-or-equal-to 80 mg/kg, but 83% of juveniles treated with greater dosages retained a visible mark. In treatments where juveniles were immersed in seawater solutions of tetracycline hydrochloride at five concentrations ranging from 200 to 1000 mg per litre of seawater and sampled at 5 h intervals for periods ranging from 5 to 40 h, all showed clear fluorescent markings. Shells of adult abalone injected with tetracycline hydrochloride at four dosages ranging from 200 to 800 mg/kg all showed clearly visible marks 18 d post-treatment. Abalone injected at dosages of 600 and 800 mg/kg exhibited tissue fluorescence around the injection site 2 wk after treatment. Adults immersed for 48 h at four concentrations ranging from 200 to 800 mg/l produced marks comparable to those of injected adults. Abalone were clearly stressed by some treatments. Only 50% of adults injected at 200 mg/kg were able to right themselves within 10 min, while all those injected at higher concentrations either were incapable of righting themselves after treatment or were extremely sluggish. All immersed adults quickly righted themselves. These results show that both injection and immersion are effective in marking abalone, but that immersion is less stressful to them.
引用
收藏
页码:81 / 86
页数:6
相关论文
共 50 条
  • [31] The effects of elevated pCO2 on growth, shell production and metabolism of cultured juvenile abalone, Haliotis iris
    Cunningham, Shaun C.
    Smith, Abigail M.
    Lamare, Miles D.
    AQUACULTURE RESEARCH, 2016, 47 (08) : 2375 - 2392
  • [32] Effects of temperature on growth of juvenile blackfoot abalone, Haliotis iris Gmelin
    Searle, Thomas
    Roberts, Rodney D.
    Lokman, P. Mark
    AQUACULTURE RESEARCH, 2006, 37 (14) : 1441 - 1449
  • [33] The role of body surfaces and ventilation in gas exchange of the abalone, Haliotis iris
    H. H. Taylor
    N. L. C. Ragg
    Journal of Comparative Physiology B, 2005, 175 : 463 - 478
  • [34] Shell boring clams in the blue abalone Haliotis fulgens and the yellow abalone Haliotis corrugata from Baja California, Mexico
    Alvarez-Tinajero, MD
    Cáceres-Martínez, J
    González-Avilés, JG
    JOURNAL OF SHELLFISH RESEARCH, 2001, 20 (02): : 889 - 893
  • [35] The Effects of Live Transport on Metabolism and Stress Responses of Abalone (Haliotis iris)
    Alfaro, Andrea C.
    Nguyen, Thao, V
    Venter, Leonie
    Ericson, Jessica A.
    Sharma, Shaneel
    Ragg, Norman L. C.
    Mundy, Craig
    METABOLITES, 2021, 11 (11)
  • [36] On-growing cultured abalone (Haliotis iris) in northern New Zealand
    Clarke, CB
    Creese, RG
    JOURNAL OF SHELLFISH RESEARCH, 1998, 17 (03): : 607 - 613
  • [37] The role of body surfaces and ventilation in gas exchange of the abalone, Haliotis iris
    Taylor, HH
    Ragg, NLC
    JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY, 2005, 175 (07) : 463 - 478
  • [38] Direct estimation of natural mortality of the New Zealand abalone, Haliotis iris
    McShane, PE
    Naylor, JR
    NEW ZEALAND JOURNAL OF MARINE AND FRESHWATER RESEARCH, 1997, 31 (01) : 135 - 137
  • [39] USE OF ALGAL DIETS TO AID EARLY WEANING IN THE ABALONE HALIOTIS IRIS
    Dyck, Matt
    Roberts, Rodney
    Jeffs, Andrew
    JOURNAL OF SHELLFISH RESEARCH, 2010, 29 (03): : 613 - 620
  • [40] The role of body surfaces and ventilation in gas exchange of the abalone, Haliotis iris
    H. H. Taylor
    N. L. C. Ragg
    Journal of Comparative Physiology B, 2006, 176 : 85 - 85