HIGH-LEVEL EXPRESSION OF ACTIVE HUMAN CYCLOOXYGENASE-2 IN INSECT CELLS

被引：43

作者：

CROMLISH, WA ^{[1
]}

PAYETTE, P ^{[1
]}

CULP, SA ^{[1
]}

OUELLET, M ^{[1
]}

PERCIVAL, MD ^{[1
]}

KENNEDY, BP ^{[1
]}

机构：

[1] MERCK FROSST CANADA INC,MERCK FROSST CTR THERAPEUT RES,DEPT BIOCHEM & MOLEC BIOL,POINTE CLAIRE H9R 4P8,PQ,CANADA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1994年 / 314卷 / 01期

关键词：

CYCLOOXYGENASE-2; OVEREXPRESSION; BACULOVIRUS; NSAIDS; GLYCOSYLATION;

D O I：

10.1006/abbi.1994.1429

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Active human cyclooxygenase-2 (Cox-2) was expressed at high levels in insect cells using a recombinant baculovirus. The specific activity of Cox-2 in the microsomes of infected cells was 0.51 mu mol O-2/min/mg and was comparable to that obtained for partially purified Cox-2 from ovine placenta (0.55 mu mol O-2/min/mg). The Cox-2 enzyme expressed in insect cells was glycosylated to varying extents and most of the cyclooxygenase activity was in the high-speed microsomal pellet. The insect-cell-expressed enzyme also showed characteristic 15-hydroxyeicosatetraenoic acid production after aspirin treatment and had typical inhibition profiles with a number of known nonsteroidal antiinflammatory drugs. (C) 1994 Academic Press, Inc.

引用

页码：193 / 199

页数：7

共 28 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

CHEN CA, 1988, BIOTECHNIQUES, V6, P632

[3] CLONING 2 ISOFORMS OF RAT CYCLOOXYGENASE - DIFFERENTIAL REGULATION OF THEIR EXPRESSION [J].