REGULATION OF ACTIVITY AND APICAL TARGETING OF THE CL-/HCO3- EXCHANGER IN RAT HEPATOCYTES

被引：112

作者：

BENEDETTI, A ^{[1
]}

STRAZZABOSCO, M ^{[1
]}

NG, OC ^{[1
]}

BOYER, JL ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,CTR LIVER,NEW HAVEN,CT 06510

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 02期

关键词：

D O I：

10.1073/pnas.91.2.792

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To test the hypothesis that rat hepatocyte canalicular Cl-/HCO3- exchange activity might be regulated by HCO3-- or protein kinase-induced changes in the apical targeting of vesicles, isolated rat hepatocytes were cultured in the presence or absence of HCO3-/CO2.Cl-/HCO3- exchange activity increased in cells cultured in the presence of HCO3-/CO2 or when stimulated by dibutyryl cAMP. Both of these effects were blocked by either colchicine or the protein kinase C agonist phorbol 12,13-dibutyrate. Fluorescence and confocal microscopy, respectively, revealed increased pericanalicular-apical membrane localization of two canalicular markers, peanut agglutinin and a 110 kDa canalicular ecto ATPase, when hepatocyte couplets were preincubated in HCO3-/CO2-containing medium, an effect that was again blocked by colchicine. Dibutyryl cAMP also stimulated canalicular localization of the 110-kDa protein. These findings suggest that hepatocyte Cl-/HCO3- exchange activity is regulated by HCO3-/CO2 and by protein kinase A and protein kinase C agonists through microtubule dependent targeting of vesicles containing this exchanger to the canalicular domain.

引用

页码：792 / 796

页数：5

共 28 条

[11] ISOLATED RAT HEPATOCYTE COUPLETS - A PRIMARY SECRETORY UNIT FOR ELECTROPHYSIOLOGIC STUDIES OF BILE SECRETORY FUNCTION [J].