NEW IMPROVED LACZ GENE FUSION VECTORS

被引：17

作者：

JAIN, C ^{[1
]}

机构：

[1] HARVARD UNIV, DEPT BIOCHEM & MOLEC BIOL, CAMBRIDGE, MA 02138 USA

来源：

GENE | 1993年 / 133卷 / 01期

关键词：

BETA-GALACTOSIDASE; CLONING VECTORS; RECOMBINANT DNA; FUSION PROTEINS; TRANSCRIPTIONAL TERMINATORS; PUC19; POLYLINKER; TRANSLATIONAL READING FRAME;

D O I：

10.1016/0378-1119(93)90231-Q

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

New plasmid vectors suitable for creating fusions with the lacZ gene have been developed. These vectors represent an improvement over currently available vectors and possess the following features: (1) an undetectable background beta-galactosidase (betaGal) activity in the absence of fusion, (2) an extended multiple cloning site (MCS), and (3) the ability to conveniently subclone in any one of three translational frames. Medium- and high-copy-number versions of these vectors have been developed.

引用

页码：99 / 102

页数：4

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