DISTRIBUTION OF SWALLOW PROTEIN IN EGG CHAMBERS AND EMBRYOS OF DROSOPHILA-MELANOGASTER

被引:0
|
作者
HEGDE, J
STEPHENSON, EC
机构
[1] UNIV ALABAMA, DEPT BIOL SCI, TUSCALOOSA, AL 35487 USA
[2] UNIV ROCHESTER, DEPT BIOL, ROCHESTER, NY 14627 USA
来源
DEVELOPMENT | 1993年 / 119卷 / 02期
关键词
MATERNAL EFFECT GENE; OOGENESIS; RNA LOCALIZATION; BICOID RNA; NUCLEAR DIVISIONS; MITOSIS; NUCLEAR MATRIX;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Drosophila maternal effect gene swallow has a role in localizing bicoid mRNA at the anterior margin of the oocyte during oogenesis, and a poorly characterized role in nuclear divisions in early embryogenesis. We have examined the distribution of swallow protein during oogenesis and embryogenesis using anti-swallow antibodies. During oogenesis, high levels of swallow protein are present in basal nurse cell cytoplasm, although small amounts are also present at the anterior oocyte margin, the site of bicoid RNA localization. Only a small fraction of swallow protein is in a position to interact directly with bicoid RNA during localization. The asymmetric distribution of swallow protein is disrupted in swallow ovaries, in which bicoid RNA becomes unlocalized late in oogenesis. swallow protein is uniformly distributed in eggs, but becomes localized to nuclei during early mitotic divisions in early embryogenesis. swallow protein enters each nucleus at the beginning of mitosis, occupies a position complementary to that of condensed chromatin, and leaves each nucleus at the end of mitosis. We show examples of nuclear division defects in swallow mutant embryos, and suggest that the abnormal nuclear divisions in early swallow embryos reflect a second function for swallow protein that contributes to abdominal segmentation defects common in swallow embryos.
引用
收藏
页码:457 / 470
页数:14
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