BINDING OF COPPER AND ZINC IONS BY AMINOACYLASE FROM ASPERGILLUS-ORYZAE

被引：0

作者：

BEDENKINA, NS ^{[1
]}

ABRAMYAN, AA ^{[1
]}

BAGDASARYAN, ZN ^{[1
]}

SHNYROV, VL ^{[1
]}

PERMYAKOV, EA ^{[1
]}

机构：

[1] REAKHROM ALL UNION CHEM REAGENTS & ULTRAPURE CHEM SUBST RES INST,YEREVAN,ARMENIA,USSR

来源：

BIOCHEMISTRY-MOSCOW | 1991年 / 56卷 / 03期

关键词：

AMINOACYLASE; PROTEIN FLUORESCENCE; MICROCALORIMETRY; ION BINDING;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The metal-binding properties of the aminoacylase from Aspergillus oryzae were investigated by methods of intrinsic tryptophan fluorescence and differential scanning microcalorimetry. At netral pH values the enzyme cooperatively binds three to four Cu2+ ions per molecule with association constant (2-9) . 10(5) M-1. Evidently Zn2+ ions are bound at the same sites. Possibly histidine residues are present in certain binding sites. The binding of Cu2+ and Zn2+ ions increases the stability of the enzyme to the action of acidic and alkaline pH but decreases its thermal stability.

引用

页码：328 / 333

页数：6

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