IDENTIFICATION AND INITIAL CHARACTERIZATION OF GLUCOSE-REPRESSIBLE PROMOTERS OF STREPTOCOCCUS-MUTANS

被引：12
作者：
LANE, MA
BAYLES, KW
YASBIN, RE
机构：
[1] UNIV MARYLAND,DEPT BIOL SCI,CATONSVILLE,MD 21228
[2] UNIV MARYLAND,PROGRAM MOLEC & CELL BIOL,CATONSVILLE,MD 21228
来源：
GENE | 1991年 / 100卷
关键词：
RECOMBINANT DNA; TRANSCRIPTIONAL FUSIONS; CATABOLITE REPRESSION; DENTAL CARIES;
D O I：
10.1016/0378-1119(91)90371-H
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Three catabolite-repressible promoters from Streptococcus mutans have been isolated. These promoters were identified by utilizing the vector pRQ200 which contains a promoterless amylase-encoding gene, a Gram- origin of replication, and an erythromycin-resistance determinant. A library of S. mutans DNA was constructed in pRQ200, amplified in Escherichia coli and integrated by Campbell-type insertion into the S. mutans chromosome following transformation. Colonies exhibiting amylase production on media lacking an extraneous carbohydrate source were screened for diminished amylase production on media containing glucose. The effect of glucose on these promoters has been characterized using a quantitative spectrophotometric assay of amylase activity.
引用
收藏
页码：225 / 229
页数：5
相关论文
共 24 条
[1]   HOW CYCLIC-AMP AND ITS RECEPTOR PROTEIN ACT IN ESCHERICHIA-COLI [J].
ADHYA, S ;
GARGES, S .
CELL, 1982, 29 (02) :287-289
[2]  
Ausubel F, 1988, CURRENT PROTOCOLS MO
[3]  
BLUMENTHAL HJ, 1972, STAPHYLOCOCCI, P111
[4]  
CARDINEAU GA, 1987, J BIOL CHEM, V262, P3344
[5]   THE ROLE OF PROTEIN-PHOSPHORYLATION IN NEURAL AND HORMONAL-CONTROL OF CELLULAR-ACTIVITY [J].
COHEN, P .
NATURE, 1982, 296 (5858) :613-620
[6]   CYCLIC-AMP RECEPTOR PROTEIN - ROLE IN TRANSCRIPTION ACTIVATION [J].
DECROMBRUGGHE, B ;
BUSBY, S ;
BUC, H .
SCIENCE, 1984, 224 (4651) :831-838
[7]  
DELLO SS, 1983, J BACTERIOL, V153, P861
[8]   PROPERTIES OF STREPTOCOCCUS-MUTANS INGBRITT GROWING ON LIMITING SUCROSE IN A CHEMOSTAT - REPRESSION OF THE PHOSPHOENOLPYRUVATE PHOSPHOTRANSFERASE TRANSPORT-SYSTEM [J].
ELLWOOD, DC ;
HAMILTON, IR .
INFECTION AND IMMUNITY, 1982, 36 (02) :576-581
[9]   BIOLOGY, IMMUNOLOGY, AND CARIOGENICITY OF STREPTOCOCCUS-MUTANS [J].
HAMADA, S ;
SLADE, HD .
MICROBIOLOGICAL REVIEWS, 1980, 44 (02) :331-384
[10]   NUCLEOTIDE-SEQUENCE AND FUNCTIONAL MAP OF PC194, A PLASMID THAT SPECIFIES INDUCIBLE CHLORAMPHENICOL RESISTANCE [J].
HORINOUCHI, S ;
WEISBLUM, B .
JOURNAL OF BACTERIOLOGY, 1982, 150 (02) :815-825
123