SIMPLE PURIFICATION OF GOAT IGG1 AND IGG2 SUBCLASSES BY CHROMATOGRAPHY ON PROTEIN A-SEPHAROSE AT VARIOUS PH

被引：38

作者：

DELACROIX, D

VAERMAN, JP

机构：

[1] Université Catholique de Louvain, International Institute of Cellular and Molecular Pathology, Unit of Experimental Medicine, B-1200 Brussels, 75, avenue Hippocrate

来源：

MOLECULAR IMMUNOLOGY | 1979年 / 16卷 / 10期

关键词：

D O I：

10.1016/0161-5890(79)90164-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two ml of goat serum are applied on a 2 × 20 cm column of protein A-Sepharose at pH 9.1. Elution at the same pH yields two protein peaks, the second being highly purified IgG1. A minor Ig fraction, eluted at pH 6.5, is discarded. Pure IgG2 is then eluted at pH 5.9. Yields of both subclasses average 70 %. © 1979.

引用

页码：837 / 840

页数：4

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