Identification of Bacteria from the Rhizosphere of Jatropha curcas with Characteristics of Biotechnological Interest

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作者
Wong-Villarreal, Arnoldo [1 ]
Kante-Lopez, Fernando [1 ]
Reyes-Reyes, Ana L. [2 ]
Sanchez-Cruz, Ricardo [1 ]
De Leon-Martinez, Antonio [1 ]
Yanez-Ocampo, Gustavo [3 ]
Espinosa-Zaragoza, Saul [4 ]
Lopez-Baez, Orlando [5 ]
Hernandez-Bueno, Nancy S. [6 ]
机构
[1] Univ Tecnol Selva, Div Agroalimentaria, Ocosingo, Chiapas, Mexico
[2] INIFAP, Campo Expt Rosario Izapa, Programa Bioenergia, Tuxtla Gutierrez, Chiapas, Mexico
[3] Univ Politecn Chiapas, Ingn Tecnol Ambiental, Tuxtla Gutierrez, Chiapas, Mexico
[4] Univ Autonoma Chiapas, Fac Ciencias Agr, Huehuetan, Chiapas, Mexico
[5] Univ Autonoma Chiapas, Ctr Univ Empresa, AUDES Cacao Chocolate, Tuxtla Gutierrez, Chiapas, Mexico
[6] Univ Autonoma Estado Morelos, Ctr Invest Biotecnol, Cuernavaca, Morelos, Mexico
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中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In the present study Pseudomonas sp. S18 (PMa-76), Pseudomonas aeruginosa N7B1 (PMa-83), Pseudomonas sp. (PMa-88), Pseudomonas nitroreducens TX1 (PMa-89), Pseudomonas strains sp. bf1 (PHx -97), Pseudomonas aeruginosa F23 (PHx -98), Pseudomonas aeruginosa F23 (PHx -101) strains were isolated from the rhizosphere of physic nut. The PMa-83 PMa-88 PMa-89, PHx -97, PHx -98, PHx -101 strains grew up in the presence of phenol 0.05% and benzene 1.0%, as a sole carbon source, as well as in the presence of zinc, copper and cobalt in concentrations of 0.1 and 5 mg/ml. The Pseudomonas sp. 818 (PMa-76) strain was the one that had the highest capacity to solubilize phosphate (9.6 mm). Moreover indole production in 6 strains were detected and while biosurfactants production was detected in 5 strains. Furthermore, the presence of nifH genes in PMa-83, PHx -97, PHx-98 and PHx P-101 strains were also detected, indicating the ability to fix nitrogen. PMa 83 is the strain in which the growth in phenol, benzene and metals were detected, thus indole production, the presence of biosurfactants and nifH genes which are characteristics of biotechnological interest.
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页码:2025 / 2032
页数:8
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