DEVELOPMENT OF AN ASSAY-METHOD FOR PURINE CATABOLIC ENZYMES IN THE MOUSE AND ITS ADAPTATION FOR USE ON AN AUTOANALYZER

被引：9
|
作者：
LETISSIER, PR
PETERS, J
SKIDMORE, CJ
机构：
[1] UNIV READING, DEPT BIOCHEM & PHYSIOL, POB 228, READING RG6 2AJ, BERKS, ENGLAND
[2] MRC, RADIOBIOL UNIT, DIDCOT OX11 0RD, OXON, ENGLAND
来源：
ANALYTICAL BIOCHEMISTRY | 1994年 / 222卷 / 01期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1006/abio.1994.1469
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An assay method has been developed for the purine catabolic enzymes adenosylhomocysteinase, adenosine deaminase (ADA), purine-nucleoside phosphorylase (PNP), and urate oxidase in mice. The assay links H2O2 produced during purine catabolism to the production of a dye complex. The assay method has been developed for ADA and PNP in erythrocytes and for all four enzymes in liver. The assay is cheap, sensitive, and easy to perform. The dye complex absorbs in the visible range, negating the need for an expensive ultraviolet spectro-photometer and allowing the use of an autoanalyzer. (C) 1994 Academic Press, Inc.
引用
收藏
页码：168 / 175
页数：8
相关论文
共 50 条
  • [21] USE OF THE ALGAL ASSAY-METHOD AND THE OXYMETER TO CHARACTERIZE THE ECOCLINE FROM AN ALNUS-GLUTINOSA WOOD TO A RAISED BOG
    TSOBEL, M
    TOOM, M
    SOVIET JOURNAL OF ECOLOGY, 1990, 21 (02) : 56 - 61
  • [22] PHARMACOKINETICS OF DILEVALOL AND ITS CONJUGATES IN MAN - ASSAY-METHOD FOR PLASMA, BLOOD, URINE AND BILE SAMPLES AND PRELIMINARY PHARMACOKINETIC STUDIES
    NEUBECK, M
    BECKER, C
    HENKE, D
    ROSCH, W
    SPAHNLANGGUTH, H
    MUTSCHLER, E
    ARZNEIMITTEL-FORSCHUNG/DRUG RESEARCH, 1993, 43-2 (09): : 953 - 957
  • [23] MANUAL PROCEDURE FOR DIRECT MICROASSAY OF SERUM GLUCOSE BY USE OF O-TOLUIDINE, AND ITS ADAPTATION TO SMA 12/60 AUTOANALYZER
    WEBSTER, WW
    STINSON, SF
    WONG, WH
    CLINICAL CHEMISTRY, 1971, 17 (10) : 1050 - +
  • [24] Radiolabeled glutathione assay: Method development and its applications
    Cai, CH
    Stankovic, C
    Gifford, E
    Surendran, N
    Cai, HL
    DRUG METABOLISM REVIEWS, 2003, 35 : 110 - 110
  • [25] IMPROVED ASSAY-METHOD OF DTMP SYNTHASE IN RAT-LIVER AND ITS APPLICATION TO HUMAN LUNG-CANCER CELLS
    HASHIMOTO, Y
    SHIOTANI, T
    FUJITA, J
    BUNGO, M
    YAMAUCHI, N
    IRINO, S
    PURINE AND PYRIMIDINE METABOLISM IN MAN VI, PT B: BASIC RESEARCH AND EXPERIMENTAL BIOLOGY, 1989, 253 : 201 - 207
  • [26] DEVELOPMENT OF AN INTACT HEPATOCYTE ACTIVATION SYSTEM FOR USE WITH THE MOUSE LYMPHOMA ASSAY
    BROCK, K
    MOORE, M
    OGLESBY, L
    ENVIRONMENTAL MUTAGENESIS, 1985, 7 : 62 - 62
  • [27] ISOLATION OF A PURINE AUXOTROPH IN MOUSE L-CELLS AND ITS USE AS A DNA TRANSFER RECIPIENT
    RAY, P
    SIMINOVITCH, L
    FEDERATION PROCEEDINGS, 1982, 41 (03) : 751 - 751
  • [28] THIAZIDES .1. LIMITATIONS OF BRATTON-MARSHALL COLORIMETRIC ASSAY-METHOD AND ITS MODIFICATIONS IN THE DETERMINATION OF CHLOROTHIAZIDE IN BIOEQUIVALENCY STUDIES
    HUNT, JP
    SHAH, VP
    PRASAD, VK
    CABANA, BE
    ANALYTICAL LETTERS PART B-CLINICAL AND BIOCHEMICAL ANALYSIS, 1980, 13 (02): : 135 - 148
  • [29] IMPROVED ASSAY-METHOD OF DTMP SYNTHASE IN RAT-LIVER AND ITS APPLICATION TO HUMAN-LUNG CANCER-CELLS
    HASHIMOTO, Y
    SHIOTANI, T
    FUJITA, J
    IRINO, S
    PEDIATRIC RESEARCH, 1988, 24 (01) : 134 - 134
  • [30] ASSAY OF IPECAC AND ITS PREPARATIONS .1. DEVELOPMENT OF METHOD
    SMITH, E
    SHARKEY, MF
    LEVINE, J
    JOURNAL OF THE ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS, 1971, 54 (03): : 609 - &
12345