OZONE INDUCTION AND PURIFICATION OF SPRUCE CINNAMYL ALCOHOL-DEHYDROGENASE

被引：56

作者：

GALLIANO, H ^{[1
]}

HELLER, W ^{[1
]}

SANDERMANN, H ^{[1
]}

机构：

[1] GSF FORSCHUNGSZENTRUM UMWELT & GESUNDHEIT GMBH,INST BIOCHEM PFLANZENPATHOL,W-8042 NEUHERBERG,GERMANY

来源：

PHYTOCHEMISTRY | 1993年 / 32卷 / 03期

关键词：

PICEA-ABIES; PINACEAE; SPRUCE; CINNAMYL ALCOHOL DEHYDROGENASE; LIGNIN BIOSYNTHESIS; OZONE;

D O I：

10.1016/S0031-9422(00)95136-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spruce needle cinnamyl alcohol dehydrogenase (CAD) was induced in seedlings and four-year-old trees two-to-four-fold by ozone concentrations that did not lead to visible damage. CAD thus acted as an early biochemical ozone marker. A highly purified CAD preparation consisting of two approximately 42 000 M(r) subunits was prepared from spruce cell cultures. A polyclonal antibody was strongly inhibitory. The enzyme had high specificity for coniferyl alcohol and NADP+, and was also detected in spruce seedlings and spruce cambial sap.

引用

页码：557 / 563

页数：7

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