A NOVEL ANTINEURONAL ANTIBODY IN STIFF-MAN SYNDROME

被引：27

作者：

DARNELL, RB

VICTOR, J

RUBIN, M

CLOUSTON, P

PLUM, F

机构：

[1] CORNELL UNIV,MED CTR,COLL MED,NEW YORK,NY 10021

[2] MEM MED CTR,SPRINGFIELD,IL 62781

[3] CORNELL UNIV,MED CTR,NEW YORK HOSP,DEPT NEUROL & NEUROSCI,NEW YORK,NY 10021

来源：

NEUROLOGY | 1993年 / 43卷 / 01期

关键词：

D O I：

10.1212/WNL.43.1_Part_1.114

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Two-thirds of stiff-man syndrome (SMS) patients harbor an autoantibody specific for a 64-kD species of glutamic acid decarboxylase (GAD), the rate-limiting enzyme in GABA synthesis. We assayed SMS antisera from two patients with SMS for the presence of anti-GAD antibodies using Western blot, immunohistochemical, and enzymatic analyses. Both SMS antisera recognized an 80-kD antigen present in human and rat neuronal extracts, and failed to recognize the 64-kD GAD species. Immunohistochemistry demonstrated neuronal binding identical to that reported with anti-GAD antibodies. Both sera depleted GAD activity from brain extracts. Our analysis indicates that these SMS antisera differ from previously reported SMS antisera by recognizing a novel 80-kD antigen, and suggests that they contain antibodies directed against either a species of GAD different in size from the 64-kD enzyme, or a protein that co-immunoprecipitates with GAD.

引用

页码：114 / 120

页数：7

共 32 条

[1] ANTIBODIES TO A 64,000 MR HUMAN ISLET CELL ANTIGEN PRECEDE THE CLINICAL ONSET OF INSULIN-DEPENDENT DIABETES [J].