INFLUENCE OF MICROSPORE DEVELOPMENT ON POLLEN PROTOPLAST ISOLATION IN DIGITALIS-OBSCURA

被引：5
作者：
ARNALTE, E
PEREZBERMUDEZ, P
CORNEJO, MJ
SEGURA, J
机构：
[1] Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, Valencia, 46010
来源：
JOURNAL OF PLANT PHYSIOLOGY | 1991年 / 138卷 / 05期
关键词：
DIGITALIS-OBSCURA; INVIVO POLLEN DEVELOPMENT; POLLEN PROTOPLAST ISOLATION;
D O I：
10.1016/S0176-1617(11)80253-1
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Protocols for the enzymatic isolation of Digitalis obscura pollen protoplasts are described. The developmental stage of the microspores was a critical factor for successful isolation since protoplasts were obtained only from tetrads. The highest yield of viable protoplasts was achieved with an enzymatic mixture containing helicase, cellulase and pectolyase in 0.5 M sucrose. In different culture techniques employed, cell wall regeneration took place only when protoplasts were plated in agarose drops.
引用
收藏
页码：622 / 624
页数：3
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