ISOLATION AND CHARACTERIZATION OF PROTECTIVE CYTOLYTIC T-CELLS IN A RODENT MALARIA MODEL SYSTEM

被引：36

作者：

ROMERO, P

MARYANSKI, JL

CORDEY, AS

CORRADIN, G

NUSSENZWEIG, RS

ZAVALA, F

机构：

[1] NYU, SCH MED, DEPT MED & MOLEC PARASITOL, NEW YORK, NY 10003 USA

[2] UNIV LAUSANNE, INST BIOCHEM, CH-1000 LAUSANNE 17, SWITZERLAND

来源：

IMMUNOLOGY LETTERS | 1990年 / 25卷 / 1-3期

基金：

美国国家卫生研究院;

关键词：

Circumsporozoite (CS) protein; Cytololytic T cells (CTL); Malaria; Plasmodium berghei; Plasmodium yoelii;

D O I：

10.1016/0165-2478(90)90086-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Protective immunity against malaria is induced by immunization with irradiation-attenuated sporozoites. Here we report the isolation of cytolytic T-cell (CTL) clones from BALB/c (H-2d) mice immunized with either Plasmodium berghei or Plasmodium yoelii sporozoites. The epitopes recognized by these CTL can be mimicked by synthetic peptides corresponding to a homologous region in the CS proteins of both rodent malaria species. Both peptides are recognized by the CTL in the context of the same MHC class I molecule, H-2 Kd. In vivo adoptive transfer of the CTL clones into non-immune syngeneic mice protected them from a lethal challenge of infectious sporozoites. © 1990.

引用

页码：27 / 32

页数：6

共 13 条

[1] PEPTIDE-SYNTHESIS .2. PROCEDURES FOR SOLID-PHASE SYNTHESIS USING N-ALPHA-FLUORENYLMETHOXYCARBONYLAMINO-ACIDS ON POLYAMIDE SUPPORTS - SYNTHESIS OF SUBSTANCE-P AND OF ACYL CARRIER PROTEIN 65-74 DECAPEPTIDE [J].