SENSITIVE AND SIMPLE METHODS FOR DETERMINATION OF L-LYSINE WITH L-LYSINE ALPHA-OXIDASE

被引：11

作者：

KUSAKABE, H ^{[1
]}

KODAMA, K ^{[1
]}

KUNINAKA, A ^{[1
]}

YOSHINO, H ^{[1
]}

SODA, K ^{[1
]}

机构：

[1] KYOTO UNIV,INST CHEM RES,MICROBIAL BIOCHEM LAB,UJI,KYOTO 611,JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1979年 / 43卷 / 08期

关键词：

D O I：

10.1080/00021369.1979.10863679

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

L-Lysine could be determined satisfactorily with a new fungal enzyme, L-lysine a-oxidase (EC 1.4.3). The method consists of the oxidative deamination of L-lysine with L-lysine a-oxidase and the spectrophotometric determination of one of the reaction products: a-keto- c-aminocaproate, its intramolecular dehydrated form, d1-piperideine-2-carboxylate or hydrogen peroxide. The method on the basis of the color reaction of hydrogen peroxide formed from L-lysine with 4-aminoantipyrine and phenol in the presence of peroxidase was most sensitive and simple. The method could be used for the direct assay of L-lysine levels in serums from several animals without pretreatments. © 1979 Taylor & Francis Group, LLC.

引用

页码：1749 / 1752

页数：4

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