ANALYSIS OF CYSTEINYLDOPAS, DOPA, DOPAMINE, NORADRENALINE AND ADRENALINE IN SERUM AND URINE USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND ELECTROCHEMICAL DETECTION

被引：134

作者：

HANSSON, C

AGRUP, G

RORSMAN, H

ROSENGREN, AM

ROSENGREN, E

EDHOLM, LE

机构：

[1] CHEM CTR,DEPT TECH ANALYT CHEM,S-22007 LUND,SWEDEN

[2] UNIV LUND,DEPT PHARMACOL,S-22101 LUND,SWEDEN

[3] UNIV LUND,DEPT BIOCHEM 1,S-22101 LUND,SWEDEN

[4] UNIV LUND,DEPT DERMATOL,S-22101 LUND,SWEDEN

[5] UNIV LUND,DEPT ORGAN CHEM 2,S-22101 LUND,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 162卷 / 01期

关键词：

D O I：

10.1016/S0378-4347(00)82058-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The catecholic amino acids, dopa, 2-S- and 5-S-cysteinyldopa, and 2,5-S,S-dicysteinyldopa were determined qualitatively in serum from patients with malignant melanoma by reversed-phase high-performance liquid chromatography, using electrochemical detection. In urine the catecholamines dopamine, noradrenaline and adrenaline were also determined qualitatively, as well as the above-mentioned compounds, in a single chromatographic run. The conditions were optimized by changing the pH of the mobile phase and by the addition of methanesulphonic acid. A comparison was made between the performance of four commercial reversed-phase packing materials containing chemically bonded octadecyl groups, using a standard mixture of catecholic amino acids. The influence of ionic strength, pH and amount of methanesulphonic acid on retention was investigated. © 1979.

引用

页码：7 / 22

页数：16

共 22 条

[1]

AGRUP G, 1976, ACTA DERM-VENEREOL, V56, P491

[2]

AGRUP G, 1976, COMMUNICATION

[3]

ANTON AH, 1964, J PHARMACOL EXP THER, V145, P326

[4] UPWARD SLURRY PACKING OF LIQUID-CHROMATOGRAPHY COLUMNS [J].