REDUCTION OF FATTY-ACID ETHYL-ESTER ACCUMULATION BY GANGLIOSIDE GM1 IN RAT FETUS EXPOSED TO ETHANOL

The biochemical mechanism of alcohol teratogenicity is not known. We have demonstrated that alcohol administration to pregnant rats during gestation days (GD) 6 and 7 and/or 13 and 14 leads to significant accumulation of ethyl esters of long chain fatty acids (FAEEs) in both maternal and fetal organs. This observation extends the report of Bearer et al. (Pediat Res 31: 492-495, 1992) who detected the presence of metabolites in maternal and fetal organs of pregnant C57Bl/6J mice exposed to alcohol on GD 7 and/or GD 14. The ethyl esters of arachidonic, linoleic, oleic, stearic and palmitic acids were major metabolites detected in both maternal and fetal organs. It was also demonstrated that detectable levels of FAEEs remain 14 days (GD 20) after initial exposure to alcohol on GD 7. Ganglioside GM1 treatment 1 and 24 hr prior to alcohol exposure on both GD 7 and/or GD 14 reduced the accumulation of FAEEs. A similar regimen was shown to prevent development of tolerance to alcohol in the adult pups exposed to alcohol in utero in our previous studies. Thus, the ganglioside GM1 may have therapeutic value in reducing neurobehavioral effects of alcohol exposure in utero.