DETERMINATION OF EPERISONE IN HUMAN PLASMA BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：9

作者：

TAKAMATSU, T ^{[1
]}

YAMAZAKI, K ^{[1
]}

KAYANO, M ^{[1
]}

TAKENAKA, F ^{[1
]}

HASUI, M ^{[1
]}

OHKAWA, T ^{[1
]}

机构：

[1] SEKISUI CHEM CO LTD,SEKISUI CLIN LAB,MISHIMA,OSAKA 618,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 584卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80584-D

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A gas chromatographic-mass spectrometric method was developed to determine eperisone hydrochloride, 4'-ethyl-2-methyl-piperidinopropiophenone hydrochloride, in human plasma over the concentration range 0.2-40 ng/ml. Excellent sensitivity was achieved by selection of a favorable fragment ion, m/z 98, of eperisone and reduction of heat decomposition of eperisone by using a splitless injector and a shortened capillary column. The method described here allows the determination of plasma concentrations as low as 0.2 ng/ml. the concentration attained 6 h after a single oral administration of 50 mg. At eperisone hydrochloride concentrations higher than 0.5 ng/ml, the mean inter-day variation of accuracy of the assay was less than 12%.

引用

页码：261 / 266

页数：6