CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDY OF MINOR GLUCOAMYLASE FROM ASPERGILLUS-AWAMORI VARIANT-X-100/D27

被引：12

作者：

GOLUBEV, AM

NEUSTROEV, KN

ALESHIN, AE

FIRSOV, LM

机构：

[1] Leningrad Nuclear Physics Institute U.S.S.R. Academy of Sciences Gatchina, Leningrad district

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 226卷 / 01期

关键词：

CRYSTALLIZATION; GLUCOAMYLASE; X-RAY;

D O I：

10.1016/0022-2836(92)90139-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of the reduced form of glucoamylase were obtained from polyethylene glycol 6000 solution by the hanging-drop method. The protein was treated with α-mannosidase to partly remove the sugar component. The crystals belong to the space group P212121 with cell dimensions a = 116.7 A ̊, b = 104.3 A ̊, c = 48.5 A ̊ and diffract beyond 2.5 Å resolution. © 1992.

引用

页码：271 / 272

页数：2

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