RECEPTOR-MEDIATED INTERNALIZATION OF FLUORESCENT CHEMOTACTIC PEPTIDE BY HUMAN-NEUTROPHILS

被引：171

作者：

NIEDEL, JE ^{[1
]}

KAHANE, I ^{[1
]}

CUATRECASAS, P ^{[1
]}

机构：

[1] DUKE UNIV,MED CTR,DURHAM,NC 27710

来源：

SCIENCE | 1979年 / 205卷 / 4413期

关键词：

D O I：

10.1126/science.472759

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tetramethylrhodamine labeled N-formyl-Nle-Leu-Phe-Nle-Tyr-Lys is a potent chemoattractant for human neutrophils. Binding of this peptide to living neutrophils was observed by means of video intensification microscopy. At 37°C, diffuse membrane fluorescence was seen initially, followed by rapid aggregation and internalization of the fluorescent peptide. These processes are dependent on specific binding to the formyl peptide chemotactic receptor.

引用

收藏

页码：1412 / 1414

页数：3

相关论文

共 50 条

[31] CALCIUM IONOPHORE, PHORBOL ESTER, AND CHEMOTACTIC PEPTIDE-INDUCED CYTOSKELETON REORGANIZATION IN HUMAN-NEUTROPHILS [J].

HOWARD, TH ;

WANG, D .

JOURNAL OF CLINICAL INVESTIGATION, 1987, 79 (05) :1359-1364

[32] MASS DETERMINATION OF RECEPTOR-MEDIATED ACCUMULATION OF PHOSPHATIDATE AND DIGLYCERIDES IN HUMAN-NEUTROPHILS MEASURED BY COOMASIE BLUE STAINING AND DENSITOMETRY [J].

AGWU, DE ;

MCPHAIL, LC ;

WYKLE, RL ;

MCCALL, CE .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 159 (01) :79-86

[33] LIGAND RECEPTOR INTERNALIZATION - A KINETIC, FLOW CYTOMETRIC ANALYSIS OF THE INTERNALIZATION OF N-FORMYL PEPTIDES BY HUMAN-NEUTROPHILS [J].

FINNEY, DA ;

SKLAR, LA .

CYTOMETRY, 1983, 4 (01) :54-60

[34] PHYSICOCHEMICAL PROPERTIES OF THE N-FORMYL PEPTIDE RECEPTOR ON HUMAN-NEUTROPHILS [J].

ALLEN, RA ;

JESAITIS, AJ ;

SKLAR, LA ;

COCHRANE, CG ;

PAINTER, RG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1986, 261 (04) :1854-1857

[35] REAL-TIME VIDEO INTENSIFICATION FLUORESCENCE MICROSCOPY OF THE DYNAMICS OF THE N-FORMYL CHEMOTACTIC PEPTIDE RECEPTOR ON HUMAN-NEUTROPHILS [J].

SCHMITT, M .

EXPERIENTIA, 1987, 43 (06) :694-694

[36] EFFECT OF PHORBOL-MYRISTATE ACETATE AND THE CHEMOTACTIC PEPTIDE FNLPNTL ON SHAPE AND MOVEMENT OF HUMAN-NEUTROPHILS [J].

ROOS, FJ ;

ZIMMERMANN, A ;

KELLER, HU .

JOURNAL OF CELL SCIENCE, 1987, 88 :399-406

[37] Receptor-Mediated NETosis on Neutrophils [J].

Chen, Tao ;

Li, Yanhong ;

Sun, Rui ;

Hu, Huifang ;

Liu, Yi ;

Herrmann, Martin ;

Zhao, Yi ;

Munoz, Luis E. .

FRONTIERS IN IMMUNOLOGY, 2021, 12

[38] PROCEDURES FOR MEASURING RECEPTOR-MEDIATED BINDING AND INTERNALIZATION OF HUMAN INTERFERON [J].

ZOON, KC ;

ARNHEITER, H ;

FITZGERALD, D .

METHODS IN ENZYMOLOGY, 1986, 119 :332-339

[39] RECEPTOR-MEDIATED INTERNALIZATION OF FLUORESCENT GONADOTROPIN-RELEASING HORMONE BY PITUITARY GONADOTROPES [J].

HAZUM, E ;

CUATRECASAS, P ;

MARIAN, J ;

CONN, PM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (11) :6692-6695

[40] CHEMOTACTIC FACTOR INACTIVATION BY STIMULATED HUMAN-NEUTROPHILS MEDIATED BY MYELOPEROXIDASE-CATALYZED METHIONINE OXIDATION [J].

CLARK, RA ;

SZOT, S .

JOURNAL OF IMMUNOLOGY, 1982, 128 (04) :1507-1513

← 1 2 3 4 5 →