RECEPTOR-MEDIATED INTERNALIZATION OF FLUORESCENT CHEMOTACTIC PEPTIDE BY HUMAN-NEUTROPHILS

被引：171

作者：

NIEDEL, JE ^{[1
]}

KAHANE, I ^{[1
]}

CUATRECASAS, P ^{[1
]}

机构：

[1] DUKE UNIV,MED CTR,DURHAM,NC 27710

来源：

SCIENCE | 1979年 / 205卷 / 4413期

关键词：

D O I：

10.1126/science.472759

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tetramethylrhodamine labeled N-formyl-Nle-Leu-Phe-Nle-Tyr-Lys is a potent chemoattractant for human neutrophils. Binding of this peptide to living neutrophils was observed by means of video intensification microscopy. At 37°C, diffuse membrane fluorescence was seen initially, followed by rapid aggregation and internalization of the fluorescent peptide. These processes are dependent on specific binding to the formyl peptide chemotactic receptor.

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页码：1412 / 1414

页数：3

相关论文

共 16 条

[1] DEMONSTRATION OF A RECEPTOR ON RABBIT NEUTROPHILS FOR CHEMOTACTIC PEPTIDES [J].

ASWANIKUMAR, S ;

CORCORAN, B ;

SCHIFFMANN, E ;

DAY, AR ;

FREER, RJ ;

SHOWELL, HJ ;

BECKER, EL ;

PERT, CB .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1977, 74 (02) :810-817

[2]

BECKER EL, 1976, AM J PATHOL, V85, P385

[3] DEMONSTRATION OF SPECIFIC C5A RECEPTOR ON INTACT HUMAN POLYMORPHONUCLEAR LEUKOCYTES [J].

CHENOWETH, DE ;

HUGLI, TE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (08) :3943-3947

[4]

HATCH GE, 1978, J EXP MED, V147, P183

[5]

ISERSKY C, 1978, J IMMUNOL, V121, P549

[6] ANTIBODIES TO PURIFIED INSULIN RECEPTOR HAVE INSULIN-LIKE ACTIVITY [J].

JACOBS, S ;

CHANG, KJ ;

CUATRECASAS, P .

SCIENCE, 1978, 200 (4347) :1283-1284

[7] DIRECT DEMONSTRATION THAT RECEPTOR CROSSLINKING OR AGGREGATION IS IMPORTANT IN INSULIN ACTION [J].

KAHN, CR ;

BAIRD, KL ;

JARRETT, DB ;

FLIER, JS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (09) :4209-4213

[8]

NIEDEL JE, J BIOL CHEM

[9] SELECTIVE NEUTROPHIL DESENSITIZATION TO CHEMOTACTIC FACTORS [J].

OFLAHERTY, JT ;

KREUTZER, DL ;

SHOWELL, HJ ;

VITKAUSKAS, G ;

BECKER, EL ;

WARD, PA .

JOURNAL OF CELL BIOLOGY, 1979, 80 (03) :564-572

[10] DEMONSTRATION OF A SPECIFIC NEUTROPHIL RECEPTOR FOR A CELL-DERIVED CHEMOTACTIC FACTOR [J].

SPILBERG, I ;

MEHTA, J .

JOURNAL OF CLINICAL INVESTIGATION, 1979, 63 (01) :85-88