TREHALASE FROM DICTYOSTELIUM-DISCOIDEUM - EVALUATION OF PRECISION AND SENSITIVITY OF CONTINUOUS, COUPLED ASSAYS

被引：8
作者：
KILLICK, KA
机构：
[1] Department of Developmental Biology, Boston Biomedical Research Institute, Boston, 02114, Massachusetts
来源：
CURRENT MICROBIOLOGY | 1979年 / 2卷 / 02期
关键词：
D O I：
10.1007/BF02603064
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Coupled polarographic, spectrophotometric, and fluorometric procedures have been developed for the assay of trehalase from the cellular slime mold Dictyostelium discoideum. Evaluation of the precision, sensitivity, and range of applicability of these assays demonstrates the superiority of these methods relative to discontinuous, colorimetric assays for enzyme purification and characterization studies. © 1979 Springer-Verlag New York Inc.
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页码：99 / 102
页数：4
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