ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) - MEASURE OF ANTIBODY CONCENTRATION OR AFFINITY

被引：193

作者：

BUTLER, JE ^{[1
]}

FELDBUSH, TL ^{[1
]}

MCGIVERN, PL ^{[1
]}

STEWART, N ^{[1
]}

机构：

[1] UNIV IOWA,COLL MED,DEPT UROL,IOWA CITY,IA 52242

来源：

IMMUNOCHEMISTRY | 1978年 / 15卷 / 02期

关键词：

D O I：

10.1016/0161-5890(78)90053-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The immune response of rats to 2,4-dinitrophenyl (DNP) following both primary and secondary immunization with DNP-BGG has been studied using five techniques for the quantitative measurement of antibody: the standard ELISA, amplified ELISA, Farr assay, precipitation and a double-antibody precipitin assay. In addition, antibody affinity was measured by a modification of the Farr assay. Results are consistent with the view that while the Farr assay is a good indicator of total antibody. as measured by double-antibody precipitation, both ELISA assays correlate better with changes in antibody affinity. The amplified ELISA is comparatively less correlated with antibody affinity than the standard ELISA and only the amplified ELISA is capable of detecting the low affinity antibody detectable by double-antibody precipitation and by the Farr assay. Because of the influence of affinity on measurements made by the ELISA. results are best expressed as ELISA units rather than μg antihody/ml. © 1978.

引用

页码：131 / 136

页数：6

共 24 条

[11] COMPARISON OF FARR TECHNIQUE WITH EQUILIBRIUM DIALYSIS FOR MEASUREMENT OF ANTIBODY CONCENTRATION AND AFFINITY [J].

KIM, YT ;

KALVER, S ;

SISKIND, GW .

JOURNAL OF IMMUNOLOGICAL METHODS, 1975, 6 (04) :347-353

[12]

LEONE CA, 1968, METHODS IMMUNOLOGY I, V3, P174

[13]

LITTLE JR, 1967, METHODS IMMUNOLOGY I, V1, P128

[14]

MANCINI G, 1963, PROTIDES BIOLOGICAL, V11, P370

[15]

MINDEN P, 1967, HDB EXPT IMMUNOLOGY, P463

[16]

PINCKARD RN, 1967, HANDBOOK EXPERIMENTA, P493

[17]

SLOAN GJ, J VET RES

[18]

SLOAN GJ, 1975, THESIS U IOWA

[19] UNLABELED ANTIBODY ENZYME METHOD OF IMMUNOHISTOCHEMISTRY - PREPARATION AND PROPERTIES OF SOLUBLE ANTIGEN-ANTIBODY COMPLEX (HORSERADISH PEROXIDASE-ANTIHORSERADISH PEROXIDASE) AND ITS USE IN IDENTIFICATION OF SPIROCHETES [J].

STERNBER.LA ;

HARDY, PH ;

CUCULIS, JJ ;

MEYER, HG .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1970, 18 (05) :315-&

[20]

STEWARD MW, 1972, IMMUNOLOGY, V23, P881

← 1 2 3 →