QUANTIFICATION OF METHANOGENIC BIOMASS BY ENZYME-LINKED IMMUNOSORBENT-ASSAY AND BY ANALYSIS OF SPECIFIC METHANOGENIC COFACTORS

被引：5

作者：

GORRIS, LGM ^{[1
]}

KEMP, HA ^{[1
]}

ARCHER, DB ^{[1
]}

机构：

[1] INST FOOD RES, NORWICH LAB, NORWICH NR4 7UA, NORFOLK, ENGLAND

来源：

BIOMASS | 1987年 / 14卷 / 03期

关键词：

BIOGAS - Production - BIOMASS - METHANE - Biosynthesis - PROCESS CONTROL;

D O I：

10.1016/0144-4565(87)90047-3

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The reliability and accuracy with which enzyme-linked immunosorbent assay (ELISA) and an assay of methanogenic cofactors detect and quantify methanogenic species were investigated. Both assays required standardization with laboratory cultures of methanogenic bacteria and were applied to mixtures of pure cultures and samples from anerobic digesters. ELISA was shown to be a simple method for detecting and quantifying individual methanogenic species. The range of species which can be assayed is limited by the range of antisera available, but, potentially, ELISA can be applied to all methanogens. Although the cofactor assay is not species-specific, it can distinguish between hydrogenotrophic and acetotrophic methanogens and it is quantitative.

引用

页码：195 / 208

页数：14

共 18 条

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