FLUORESCENT IN-SITU HYBRIDIZATION AS AN AID TO INTRODUCING ALIEN GENETIC-VARIATION INTO WHEAT

被引：11

作者：

MILLER, TE ^{[1
]}

READER, SM ^{[1
]}

PURDIE, KA ^{[1
]}

ABBO, S ^{[1
]}

DUNFORD, RP ^{[1
]}

KING, IP ^{[1
]}

机构：

[1] UNIV READING,DEPT AGR BOT,READING RG6 2AS,BERKS,ENGLAND

来源：

EUPHYTICA | 1995年 / 85卷 / 1-3期

关键词：

ALIEN INTRODUCTION; FLUORESCENT IN SITU HYBRIDIZATION (FISH); WHEAT;

D O I：

10.1007/BF00023956

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Fluorescent in situ hybridization (FISH) has been used to assess the occurrence and frequency of wheat-alien chromosome pairing in a wheat/Thinopyrum bessarabicum hybrid and in wheat/rye hybrids with different levels of chromosome pairing by examining pollen mother cells at metaphase I of meiosis. The use of FISH to identify the presence and size of alien chromatin in a wheat background is also demonstrated. The value of FISH as an aid to the introgression of alien genetic variation into wheat is discussed.

引用

页码：275 / 279

页数：5

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