IMPROVED VIRUS SAFETY AND PURITY OF A CHROMATOGRAPHICALLY PRODUCED FACTOR-IX CONCENTRATE BY NANOFILTRATION

被引：31
|
作者：
HOFFER, L
SCHWINN, H
BIESERT, L
JOSIC, D
机构：
[1] OCTAPHARMA PHARMAZEUT PROD MBH,A-1100 VIENNA,AUSTRIA
[2] GEORG SPEYER HAUS,D-60596 FRANKFURT,GERMANY
来源：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 669卷 / 02期
关键词：
D O I：
10.1016/0378-4347(95)00107-T
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A virus removal system based on tangential flow filtration was introduced into a Factor IX production process. Beside the intended virus reduction potency of filter membranes, an additional purification effect could be achieved. This purification effect was evaluated in detail by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis and size-exclusion HPLC. High-molecular-mass impurities were retained by the membrane, thus increasing the specific activity of the product.
引用
收藏
页码：187 / 196
页数：10
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