LACTOSYLCERAMIDE IS SYNTHESIZED IN THE LUMEN OF THE GOLGI-APPARATUS

被引：82

作者：

LANNERT, H ^{[1
]}

BUNNING, C ^{[1
]}

JECKEL, D ^{[1
]}

WIELAND, FT ^{[1
]}

机构：

[1] UNIV HEIDELBERG, INST BIOCHEM 1, D-69120 HEIDELBERG, GERMANY

来源：

FEBS LETTERS | 1994年 / 342卷 / 01期

关键词：

GLYCOSPHINGOLIPID; LACTOSYLCERAMIDE SYNTHASE; GOLGI; SEMIINTACT CELL;

D O I：

10.1016/0014-5793(94)80591-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, synthesis of lactosylceramide has been described to occur on the cytosolic face of the Golgi [(1991) J. Biol. Chem. 266, 20907-20912]. The reactions following in the biosynthesis of higher glycosphingolipids are known to take place in the lumen of the Golgi. For our understanding of the functional organization of the multiglycosyltransferase system of glycosphingolipid synthesis in the Golgi, the knowledge of the topology of individual reactions is a prerequisite. We have developed a simple and quick assay system for sphingolipid biosynthesis and have obtained evidence that lactosylceramide is synthesized in the lumen of the Golgi. Because lactosylceramide is generated by galactosylation of glucosylceramide which, in turn, is synthesized from ceramide and UDP-Glc on the cytosolic surface of the Golgi apparatus, further efforts will be directed to the characterization of a glucosylceramide-translocator in the Golgi membranes rather than a lactosylceramide-translocator.

引用

页码：91 / 96

页数：6

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